Blend with an rising level of ondansetron HCl. Measurement of film thickness Thickness of every film was measured working with thickness gauze (Mitutoyo, Japan). The measurement of thickness of every film was accomplished at six distinct places (two in middle portion and fourMATERIALS AND METHODSHPMC E5 and HPMC K100 had been obtained exgratis from Corel Pharm Ltd. (Ahmedabad, India). EudragitNE 30 D was obtained from R m Gmbh, Germany. Ondansetron hydrochloride wasTable 1: Composition of the polymeric ondansetron filmsFormulation code F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F5a F8aaHPMC E5 (mg) 1,000 1,000 1,000 1,000 1,000 1,250 1,000 1,000 1,000 1,250 1,000 1,HPMC K100 (mg) 500 1000 500 500 750 500 750 500 500 500 750Eudragit NE 30 D (mg) 250 500 500 500 750 750 1000 1000 500Ondansetron HCl (mg) 150 150 150 150 150 150 150Propylene glycol 100 100 one hundred one hundred one hundred 100 one hundred one hundred 100 one hundred 100Denotes placebo films, HPMC: Hydroxypropyl methylcelluloseTable 2: Composition of polymeric films with varying drug concentrationFormulation code F1 F11 F12 F13 HPMC E5 (mg) 1,000 1,000 1,000 1,000 HPMC K100 (mg) 500 500 500 500 Eudragit NE 30 D (mg) Ondansetron HCl (mg) 78 115 150 Propylene glycol 100 one hundred 100HPMC: Hydroxypropyl methylcelluloseInternational Journal of Pharmaceutical Investigation | April 2013 | Vol 3 | IssueKumria, et al.: Buccoadhesive polymeric films of ondansetroncorners). For every formulation, three randomly chosen films had been utilised. Films of size (3 3 cm2) have been cut and all the measurements have been completed in triplicate. Mean worth of film thickness at six diverse areas was taken as the film thickness.[12] Determination of drug content material inside the films To ensure the uniformity of distribution of ondansetron inside the film, a content material uniformity test was done. Films (1 1 cm2 equivalent to 2 mg of ondansetron) were reduce at 3 distinct areas and dissolved in 10 ml of phosphate buffer saline (pH six.8) by continuous shaking on a water bath at space temperature for 8 h.[13] The option was filtered by means of Whatman filter paper plus the samples have been diluted suitably and analyzed making use of UV spectrophotometer at a max 310 nm against a blank (UV1800, Double Beam spectrophotometer, SHIMADZU, Japan). A calibration curve was constructed along with the drug content material was estimated in the curve (two.520 /ml). The method validation was performed for linearity, precision, and accuracy.ML115 The regression equation for the calibration curve was Y = 0.Tetracycline 041X + 0.006; R2 = 0.9990. Folding endurance Folding endurance value was calculated by folding the film of suitable size in the same place and counting the amount of time the film might be folded with no breaking.PMID:28440459 [14] Swelling study: Percentage of hydration and matrix erosion Film swelling properties and erosion traits had been determined by calculating the percentage of hydration and matrix erosion on the films. Films of definite size (1 1 cm2) had been reduce and weighed (W1). Film was placed on a weighed stainless steel wire mesh. The wire mesh and the film had been immersed in phosphate buffer saline (pH 6.eight) for predetermined time periods (5, ten, 20, 40, 60, 90, 120 min). At these time intervals the wire mesh was withdrawn in the buffer, the films had been wiped off working with filter paper and weighed (W2). Percentage hydration with the films was determined making use of the following relation:[15] Percentage of hydration = W2 W1/W2 one hundred Just after complete hydration, films have been dried at 60 for 24 h and placed in desiccators for 48 h. The dried fil.