S-NATs had been identified by RT-PCR amplification. In all, 189,348 smaller RNAs, 27,465 of which were distinctive, were derived from 6808 NATs. These compact RNAs have been found to become enriched in the overlapping regions of NATs. The usage of deep sequencing of the degradome is broadly applicable for international identification of little RNA targets [27-30]. Analyses in the soybean degradome database [31,32] identified 446 genes because the targets of 165 nat-siRNAs in soybean. Furthermore, we detected five trans-NAT transcripts that can be folded in to the stem-loop structures which are characteristic of premiRNAs, and identified 86 targets of soybean miRNA that contained antisense transcripts in soybean.Quantity of transcriptsResults and discussionPrediction of NATs in soybeanWe analyzed 66,213 soybean transcripts downloaded from the Phytozome database (http://www.phytozome. net/index.php) [26]. More than 13 (eight,634) with the transcripts had at the very least one particular antisense transcript in soybean. Among these transcripts, more than 50 (four,788) had only a single antisense transcript, although the other people had from two to 177 antisense transcripts (Figure 1). A total of 26,216 NATs were identified in soybean. The NATs had been categorized into cis-NATs and trans-NATs as outlined by the transcript origin from the genomic loci. Mapping on the NAT transcripts to the soybean genome identified 994 cis-NATs and 25,222 trans-NATs (Added files 1 and two).Frequency ( )80 60 40 20 0 cis-NATs trans-NATs100-199 200-299 300-399 400-499 500-599 Overlap length (bp)Figure 2 Length distribution inside the overlapping regions of cis-NATs (black) and trans-NATs (white).GSK1059615 Cancer Zheng et al. BMC Genomics 2013, 14:280 http://www.biomedcentral/1471-2164/14/Page 3 ofTable 1 Genes forming cis-NATs with multiple antisense transcriptsGene 1 Gene two Glyma02g14430.1 Glyma02g36840.1 Glyma02g36830.1 Glyma02g36850.1 Glyma04g34480.1 Glyma04g34490.1 Glyma04g34500.Ouabain Autophagy 1 Glyma05g22920.1 Glyma05g22910.1 Glyma05g22930.1 Glyma08g01140.1 Glyma08g01150.1 Glyma08g01160.1 Glyma13g11820.1 Glyma13g11830.1 Glyma13g11840.1 Glyma13g11850.1 Glyma13g11860.1 Glyma13g11870.1 Glyma13g11880.1 Glyma13g11890.1 Glyma13g11900.1 Glyma13g11910.1 Glyma13g11920.1 Glyma13g11940.1 Glyma13g11950.1 Glyma13g11960.1 Glyma13g11970.1 Glyma15g37560.1 Glyma15g37570.1 Glyma15g37580.1 Glyma17g31520.1 Glyma17g31510.1 Glyma17g31530.1 Glyma17g36410.1 Glyma17g36400.1 Glyma17g36420.1 Glyma18g03060.1 Glyma18g03050.1 Glyma18g03070.Overlap length (bp) 216 333 149 53 62 113 118 63 69 1932 428 593 428 230 192 186 162 271 118 72 147 118 542 69 45 137 118 677 304 58TypeGlyma02g14410.1 Glyma02g14420.enclosed convergent divergent convergent enclosed convergent divergent enclosed enclosed convergent divergent enclosed enclosed enclosed enclosed enclosed enclosed enclosed enclosed enclosed enclosed enclosed enclosed enclosed divergent convergent divergent enclosed divergent convergent divergent31,808 bp (308 bp typical), whereas the trans-NAT overlaps length ranged from 31,716 bp (87 bp average).PMID:34235739 The overlapping length of the majority of transNATs (74.87 ) was shorter than 100 bp, and only 7.31 were longer than 200 bp (Figure 2). Several transcripts have a number of antisense transcripts in plant. For the cis-NATs, various genes are involved in two cis-NATs in Arabidopsis [11]. In soybean, we identified 11 transcripts that formed two or far more cis-NATs with other transcripts (Table 1). Glyma13g11820.1 and Glyma13g11940.1 had ten and three antisense transcripts respectively. The significant genomic sequence sizes of Glyma13g11.