Olcs Rudn , Sruthy Maria Augustine and Kaushal Kumar Bhati Received: 28 November 2022 Revised: ten January 2023 Accepted: 12 January 2023 Published: 15 JanuaryAbstract: Haberlea rhodopensis is a exclusive resurrection plant of higher phenotypic plasticity, colonizing both shady habitats and sun-exposed rock clefts. H. rhodopensis also survives freezing winter temperatures in temperate climates. Though survival in conditions of desiccation and survival in conditions of frost share high morphological and physiological similarities, proteomic adjustments lying behind these mechanisms are hardly studied. As a result, we aimed to reveal ecotype-level and temperature-dependent variations within the protective mechanisms by applying each targeted and untargeted proteomic approaches. Drought-induced desiccation enhanced superoxide dismutase (SOD) activity, but FeSOD and Cu/ZnSOD-III were drastically much better triggered in sun plants. Desiccation resulted within the accumulation of enzymes involved in carbohydrate/phenylpropanoid metabolism (enolase, triosephosphate isomerase, UDP-D-apiose/UDP-D-xylose synthase two, 81E8-like cytochrome P450 monooxygenase) and protective proteins like vicinal oxygen chelate metalloenzyme superfamily and early light-induced proteins, dehydrins, and smaller heat shock proteins, the latter two usually getting located in the newest phases of dehydration and being extra pronounced in sun plants. Even though low temperature and drought stress-induced desiccation trigger related responses, the organic variation of those responses in shade and sun plants calls for interest for the pre-conditioning/priming effects that have higher value both within the desiccation responses and productive strain recovery. Keyword phrases: drought pressure; frost-induced desiccation; LC-MS/MS; proteomics; resurrection plants1. Introduction Resurrection plants represent a little group of angiosperms that possess the capability to survive desiccation to an air-dry state and recover to typical metabolic functions upon rehydration [1]. Haberlea rhodopensis, like other European species which include Ramonda serbica, Ramonda nathaliae, Ramonda myconi, and Jankaea heldreichii, is often a desiccation-tolerant member on the Gesneriaceae family [2]. The homoiochlorophyllous resurrection plant H. rhodopensis can be a Tertiary relict around the Balkan Peninsula [6,7].Anti-Mouse IFNAR1 Antibody Epigenetic Reader Domain In its organic habitat in the Rhodope Mountains, H.Turkesterone MedChemExpress rhodopensis colonizes rock surfaces at an altitude from 136 to near 1600 m a.PMID:24576999 s.l., along with the sites of occurrence particularly differ in temperature, humidity, and light circumstances [8]. Even though the taxon features a clear preference for shady habitats (e.g., north-faced limestone or undercanopy silicate rocks and maximum photosynthetically active photon flux density (PPFD) of 250 ol m-2 s-1 ; these are known as shade plants), H. rhodopensis also colonizes rock clefts directly exposed to sunlight (maximum PPFD of 1500700 ol m-2 s-1 ; referred to as sun plants). In the natural habitat, each shade and sun plants undergo desiccation in response to drought strain because of the lack ofCopyright: 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is definitely an open access post distributed below the terms and conditions of your Creative Commons Attribution (CC BY) license ( creativecommons.org/licenses/by/ four.0/).Plants 2023, 12, 401. doi.org/10.3390/plantsmdpi/journal/plantsPlants 2023, 12,2 ofprecipitation through the summer time [9,10]. Moreover, in contrast to most resurrection plants of tropical/subtropi.