Odies in relevant in vitro models. Coupling immune-isolation to pre-analytical protocols for urine processing and sample good quality testing enables detection of a panel of endometrial genes in urine-recovered EVs. Summary/Conclusion: General, the study provides a tool for non-invasive monitoring on the functional status of the mGluR4 review endometrium, supporting biomedical niches like assisted fertilization and diagnosis of endometriosis. Funding: ENDEvor POR Area Tuscany (identification from the project) and Exosomics R D ProgrammePF09.Unveiling autologous blood doping: comparative evaluation of various purification methods for urinary extracellular vesicles pioneering miRNA biomarker investigation Veronika Mussacka, Georg Wittmannb and Michael Pfafflc TUM College of Life Sciences Weihenstephan, Division of Animal Physiology and Immunology, Freising, Germany, Freising, Germany; bDepartment for Transfusion Medicine, Cell therapeutics and Haemostaseology, University Hospital LMU, Munich, Germany, M chen, Germany; cAnimal Physiology and Immunology, College of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany, Freising, Germanyafive distinct isolation tactics have been evaluated: ultracentrifugation, membrane affinity, spin column chromatography, immunoaffinity and precipitation. Following EV characterization by nanoparticle tracking evaluation, western blotting, and transmission electron microscopy, total RNA was isolated and a library for little RNA sequencing was prepared. The resultant productive method was then applied to each of the collected samples which were equally analysed regarding their EV distribution and miRNA content. Final results: The comparative analysis disclosed substantial discrepancies with respect to EV yield, population, and purity, also as RNA yield and detected miRNAs. By applying the top performing strategy, which was according to immunoaffinity, considerably higher amounts of urinary EVs and various drastically differentially regulated miRNAs have been observed just after ABD. Summary/Conclusion: Urinary EVs and their miRNA profile hold certainly promising attempts for the clear separation of ABD and non-doped athletes. In addition, the integrated complicated comparative methodological analysis contributes enormously to future standardization and comparability of urinary EV analysis. Funding: The present project has been financially supported by the WADA.PF09.Extracellular vesicles as graft biomarkers to address lung transplantation outcome Mario Barilania, Ilaria Righib, Giuseppe Buonoc, Lorenzo Rossod, Mario Nosottie and Lorenza Lazzarica Unit of Regenerative Medicine Cell Factory, Division of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy; Universitdegli Studi di Milano, Milan, Italy; bThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; cUnit of Regenerative Medicine Cell Factory, Division of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; dUniversitdegli Studi di Milano, Milano (MI), Italy, Milano, ItalyIntroduction: Autologous blood doping (ABD) increases the oxygen capacity by means of re-infusion of a person’s autologous red blood cells. It can be consequently utilised by endurance athletes having a high amount of unreported situations, especially due to the fact mGluR5 Storage & Stability trusted methods for unequivocal detection are still lacking. To su.