Ults: We found that the mucus layer of VSG animals was enriched with high-density nanoparticles relative to sham-operated animals. Broadly, we discovered that mucus-based EV proteins largely originate from the host and that VSG EVs have far more protein diversity, in spite of originating from fewer external species relative to sham animals. Remedy of modest intestinal enteroids with mucosal EVs from shamoperated animals resulted in increased survival relative to untreated enteroids. Surprisingly, therapy with EVs from VSG-operated animals was highly cytotoxic. When we repeated remedy in enteroids originating from Tlr4-/- mice, the cytotoxicity of VSG EVs remained, indicating that microbial EV-based LPS didn’t induce the observed cytotoxicity by means of established Tlr4 pathways. Summary/Conclusion: These preliminary results demonstrate that the mucus layer of obese animals are substantially altered by metabolic surgery and that mucosal EVs may possibly play a vital role in regulating intestinal epithelial physiology, especially for the duration of the weeks following metabolic surgery. Funding: This perform was supported by grants from NIH/NIDDK (R01DK107652, T32DK101357).PS06.Human bone marrow mesenchymal stem cells derived extracellular vesicles in vitro characterization and cytotoxicity on rat islet cells Sara Assar Kashani1; Faezeh Shekari2; Ensiyeh Haji Zadeh1 Royan institute, Tehran, Iran; 2Department of Stem Cells and Developmental Biology, Cell Science Study Center, Royan Institute for Stem Cell Biology and Technologies, Tehran, IranBackground: Exosomes are able to transfer antioxidant Complement Component 1s Proteins Source enzymes, namely catalase and superoxide dismutase. Besides, circulating exosomes can generate reactive species throughout pathological situations. Lately it was demonstrated that healthful ageing process induces a disruption on circulating exosomes, particularly CD63 content material, and an oxidative profile with improved reactive species levels. Our aim was to investigate the impact of ageing course of action on catalase SARS-CoV-2 E Proteins Recombinant Proteins activity in circulating extracellular vesicles. Strategies: The Regional Ethics Committee (CEUA Comiss de ica no Uso de Animais UFRGS; nr. 29.818) approved all animal procedures and experimental conditions. Male Wistar rats of 3- and 21-month-old were utilized. Extracellular vesicles had been obtained applying a commercial kit based on vesicles precipitation (ExoQuick, System Biosciences). The samples had been incubated with ethanol (ten) and Triton (10). The catalase activity was evaluated at 25 in order to ascertain the rate of degradation of H2O2 in 10 mM phosphate buffer (pH 7.0) at 240 nm. The catalase activity was normalized for total protein content. Results: Circulating extracellular vesicles of aged rats showed lowered catalase activity compared to young adult groups. Summary/Conclusion: Our results recommend that the normal ageing method can modify the capacity of circulating extracellular vesicles for molecules exchange, for instance antioxidant enzymes. Funding: This function received monetary assistance from Conselho Nacional de Desenvolvimento Cient ico e Tecnol ico-CNPq (grant# 476634/ 2013-01). CNPq fellowships (Dr. I.R. Siqueira; K. Bertoldi; L.R. Cechinel).PS06.Plasmatic extracellular vesicles released in days through high particulate matter levels are able to activate endothelial cells Federica Rota1; Chiara Favero2; Rita Antonioli2; Laura Pergoli2; Laura Cantone2; Mario Barilani2; Lorenza Lazzari3; Valentina Bollati1 EPIGET LAB, Division of Clinical Sciences and Community Wellness, Unive.