ly different involving the high-yielding strain (H) as well as the low-yielding strain (L) inside the three modules of blue, brown, and bisque4. The outcomes of correlation evaluation involving two modules (Supplementary Figure S4) show that blue and brown, and blue and bisque4 are substantially negatively correlated, with correlation coefficients of – 0.7 and – 0.59, respectively. Brown and bisque4 are weakly correlated, with a correlation coefficient of 0.24. GO and KEGG enrichment analysis on blue, brown, and bisque4.GO enrichment analysis was carried out on genes PI3Kγ Synonyms within the three modules of blue, brown, and bisque4, respectively (Supplementary Figure S5). The results showed that genes in these three modules were mostly enriched in catalytic activity and binding in the molecular functions; metabolic processes, cellular processes, and single-organism processes in the biological processes; and cell and cell parts within the cellular component. The three modules had precisely the same GO enrichment results, only the amount of genes was diverse. Moreover, KEGG enrichment outcomes (Supplementary Table S3) for the 3 modules were not exactly the same. The brown module (P 0.05) was primarily enriched inside the metabolic pathways of glyceride, sulfur, and galactose; non-homologous end joining; and microbial metabolism in diverse environments (Fig. 3). The blue module (P 0.05) was mainly enriched inside the metabolism and biosynthesis of different amino acids; metabolism of oxycarboxylic acid, and folate; biosynthesis of secondary metabolites, aminoacyl-tRNA, pantothenate, and CoA; microbial metabolism in diverse environments; basalResultsScientific Reports | Vol:.(1234567890)(2021) 11:18207 |doi.org/10.1038/s41598-021-97616-nature/scientificreports/Figure 1. Venn diagrams of DEGs. (a) Venn diagram of DEGs through 3 cultivation periods of high-yielding strains; (b) Venn diagram of DEGs in the course of three cultivation periods in the low-yielding strains; (c) Venn diagram of DEGs between high-yielding and low-yielding PKCζ Purity & Documentation strains in three culture periods; (d) Venn diagram of DEGs in high-yielding strains, low-yielding strains, amongst high- and low-yielding strains. transcription elements, and so on. (Fig. 4). The bisque4 module (P 0.05) was mostly enriched within the cell cycle; meiosis; DNA repair; mismatch repair; nucleotide excision repair; base excision repair; biosynthesis of terpenoid backbones, and unsaturated fatty acids; and fatty acid metabolism (Fig. 5). The KEGG enrichment outcomes of your 3 modules have been drastically various, which was constant together with the final results of your module correlation analysis. The genes related to triterpenoid anabolism in every single module have been chosen as outlined by KEGG annotation benefits of genes, and those genes together with the above gene’s expression correlation weight worth amongst inside the module have been top rated 10 had been chosen (Supplementary Table S4). Those genes had been selected for GO and KEGG enrichment. GO enrichment (Supplementary Figure S6) showed that these chosen genes have been mostly concentrated in catalytic activity and binding within the molecular functions; metabolic processes, cellular processes, and single-organism processes in the biological processes; and cell and cell parts in the cellular element. The enrichment of those 3 modules’ genes was still fundamentally precisely the same. Detailed GO information and facts of those 3 modules’ genes is displayed in Supplementary Tables S5. KEGG enrichment benefits of genes related to triterpenoid biosynthesis in every module (Supplementary Table S8