Des. Worms have been permitted to migrate for 50 min, then counted in the 1 cm drawn circle about the respective odorants. Assays were run in triplicates. Information are expressed as the option index given within the figure legends.Statistical analysisPhotographs of animals on meals leaving and on survival plates were carried out by an Olympus SZ61-Tr stereomicroscope with a Greenough optical system, below dark-field illumination with 0.67.5magnifications and also a CAM-EP50 5Mpx Camera. Evaluation and quantification of IL-8 manufacturer fluorescence were carried out as previously described [16], with modifications. Following therapies, at least 20 worms per condition had been picked individually and immobilized by 20 mM NaN3 washed in M9 buffer onto a 2 agarose pad. Microscopic examination was carried out on a NIKON Eclipse E400 type fluorescence microscope linked to a Diagnostic Instruments SPOT 500 MCT4 Species camera in case of TJ356, TJ375, CY573, MJCU017, LD1171, SJ4005, and CF1553 strains and OLYMPUS CKX53 Fluorescence microscope, OLYMPUS DP74 Cooled colour camera in case of LD001 strain, applying green fluorescent filters. Photos are representatives of no less than 3 independent experiments, except Fig. S3. Fluorescence intensity measurements have been quantified with ImageJ. Visualization of SKN-1::GFP nuclear punctae have been carried out by the OLYMPUS CellSens v2.3 Imaging application.Chemotaxis assayKaplan eier log-rank tests working with the program IBM SPSS Statistics have been carried out to evaluate toxicity assays. Meals avoidance and chemotaxis assays have been examined by one-way ANOVA with Fisher’s LSD post hoc test. Odor preference assays were analyzed by two-way ANOVA with Fisher’s LSD post hoc test just after evaluation of regular distribution significance by the Shapiro-Wilk test. Significance in fluorescence intensity was calculated by unpaired Student’s t test following evaluation of regular distribution significance by the Kolmogorov-Smirnov test. One-way ANOVA with Fisher’s LSD post hoc tests, Shapiro-Wilk and Kolmogorov-Smirnov tests, and unpaired Student’s t test were carried out working with IBM SPSS Statistics, whilst two-way ANOVA with Fisher’s LSD post hoc tests were performed with STATISTICA. Information were expressed as mean typical error of your imply (SEM). Statistical levels of significance are shown inside the figures as follows: n.s., not substantial; p 0.05; p 0.01; p 0.001.Supplementary InformationThe on the internet version includes supplementary material accessible at https://doi. org/10.1186/s12915-021-00956-y. Further file 1: Supplementary Techniques. Supplementary Approaches Kinetic Chemotaxis, Motility and Thermotolerance Assay. Fig. S1. Undiluted BA and DA on kinetic chemotaxis and thermotolerance. Fig. S2. Odor preconditioning-induced motility and aversion. Fig. S3. ccBA on DAF-16 translocation and expression of many tension reporters. Fig. S4. Impact of daf-16 and skn-1 RNAi on ccBA survival. Fig. S5. Toxicity and meals aversion by undiluted methyl-salicylate. Fig. S6 ccBA impact on food avoidance of sgk-1 and pmk-1 mutants. Additional file two. : Raw data and statistics to Figs. 1e, f and S1d and fluorescence data to Fig. 3f. Acknowledgements We thank the Caenorhabditis Genetics Center, T. Keith Blackwell, Johji Miwa and Tibor Vellai for the C. elegans strains; T. Keith Blackwell, Keith P. Choe, and Eileen Devaney for the RNAi strains; and Wormbase for collecting and offering the data on C. elegans. We’re grateful to Beatrix Gil yi and Mil Somogyv i for the technical and methodological help, Szilvia Bl esi for.