F SOD (Cu/ Zn, Mn and FeSOD). The animals were examined
F SOD (Cu/ Zn, Mn and FeSOD). The animals were examined by palpation during the study to characterize the time course of tumor development. At sacrifice (at 20 weeks of rats’s age) tumorsTable 1 Composition of diets administered of different groupsDiets DMBA + 1 1. Standard diet 2. Zinc 6.9 mg/mL (231 mg Zn/kg diet) 3. Zinc 6.9 mg/mL (231 mg Zn/kg diet) and resveratrol 0.1 mg/mL (0.2 mg/kg bw) 4. Zinc (6.9 mg/mL) (231 mg Zn/kg diet) and genistein 0.1 mg/mL (0.2 mg/kg bw) 5. Copper 1.3 mg/mL (42.6 mg Cu/kg diet) 6. Copper 1.3 mg/mL (42.6 mg Cu/kg diet) and resveratrol 0.1 mg/mL (0.2 mg/kg bw) + 2 + + 3 + + 4 + + 5 + + 6 + + 7 + 8 + + 9 + + groups/FCCP cost carcinogens DMBA 10 + + 11 + + 12 + +Abbreviations: DMBA – 7,12-dimethylbenz[a]anthracene; DMBA (+) PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27689333 corresponds to DMBA-treated groups; DMBA (-) corresponds to DMBA-untreated groups.Bobrowska et al. Lipids in Health and Disease 2011, 10:40 http://www.lipidworld.com/content/10/1/Page 3 ofwere removed, weighed and histopathological examinaton was performed. The results obtained were statistically assessed using the Kolmogorow-Smirnow test, ANOVA with POSTHOC NIR tests (SPSS 12 programme).Results and discussion Mammary glands of several rat strains, mainly SpraqueDawley, are susceptible to transformation induced by chemical carcinogens. One widely used active chemical inductors of mammary carcinogenesis is 7,12-dimethylbenz[a]anthracene [1]. The results of this study indicated that the amount of 8-isoPGF 2a in serum and urines from rats treated with cancerogenic factor and receiving a standard diet was higher than in the control group receiving analogous diet but with no DMBA treatment, however, the differences were not statistically significant for the reason of high values of standard deviation (Figures 1, 2 and 3). Similar trend regarding amount of 8-isoPGF2a in serum of rats treated with carbon tetrachloride was acquired by Kadiiska et al. [12]. Administration of DMBA resulted in an increase of biomarkers indicating damage of DNA (8-hydroxy-2’deoxyguanosine), proteins (carbonyl groups of amino acids) and lipids (8-isoPGF2a) [13]. These findings may confirm the relationship between DMBA and stimulation of the free radical processes. It was found that zinc administration combined with selected polyphenolic compounds (resveratrol or genistein) statistically decreased the biomarker content level inthe rat serum and urine compared with the animals receiving exclusively a standard diet (p < 0,05) (Figures 1, 2 and 3). It was demonstrated that diet supplementation with zinc did not decrease the content level of the studied biomarker in serum of DMBA-treated rats. On the contrary, it stimulated the process (853,576 ?428,232 versus 897,979 ?400,809 pg/ml), although, the response was not statistically significant (p < 0,05). In this case, synergistic reactions between Zn and polyphenols was observed. Evidence shows an ability of polyphnols to scavange free radicals, build complexes with metal ions catalysing formation of free radicals, and also inhibiting certain enzymes participating in oxidation [6-8]. Zn plays an essential role in cell membrane integrity and is a component of more than 300 different enzymes that function in many aspects of cellular metabolism. In numerous systems Zn can antagonize the catalytic properties of the redox-active transition metals (Fe and Cu) with respect to their abilities to promote formation of hydroxyl radical (.OH) from H2O2 and superoxide [14]. Surprisingly, the resu.