Materials and Methods Cultivation of E. chaffeensis Cultivation of ehrlichiae was performed in DH Protein Sample Preparation for For To March E. chaffeensis TRP protocol at Cloning and Expression of Recombinant E. chaffeensis TRPFor recombinant TRP Sample Preparation for Mass Spectrometry The protein of interest was excised from the described
r chambers. To determine whether T-cell migration was G protein-mediated, the cells were pre-incubated with 100 ng/ml pertussis toxin for 1 h at 37uC, washed, and then loaded in the…