Based on this idea, one particular can use this technique to quickly observe the motion of a single pathogen or pathogen mimetic prior to internalization and the time study course of acidification on internalization (i.e. the time essential for the fluorescence drop)

Desk 1. Calculated t1/two (minutes) of InlA-bead internalization, phagosomal acidification and phagosomal-endosomal/ lysosomal fusion in MDCK and Caco-2 cells.utmost price of ,.eighty for MDCK and Caco-2 cells, respectively. This maximal…